期刊
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
卷 97, 期 15, 页码 7061-7071出版社
SPRINGER
DOI: 10.1007/s00253-013-5027-2
关键词
Escherichia coli; Fatty alcohol; Fatty acyl-CoA reductase; Marinobacter aquaeolei VT8
资金
- 100-Talent Program of the Chinese Academy of Sciences foundation [O91001110A]
Although successful production of fatty alcohols in metabolically engineered Escherichia coli with heterologous expression of fatty acyl-CoA reductase has been reported, low biosynthetic efficiency is still a hurdle to be overcome. In this study, we examined the characteristics of two fatty acyl-CoA reductases encoded by Maqu_2220 and Maqu_2507 genes from Marinobacter aquaeolei VT8 on fatty alcohol production in E. coli. Fatty alcohols with diversified carbon chain length were obtained by co-expressing Maqu_2220 with different carbon chain length-specific acyl-ACP thioesterases. Both fatty acyl-CoA reductases displayed broad substrate specificities for C12-C18 fatty acyl chains in vivo. The optimized mutant strain of E. coli carrying the modified tesA gene and fadD gene from E. coli and Maqu_2220 gene from Marinobacter aquaeolei VT8 produced fatty alcohols at a remarkable level of 1.725 g/L under the fermentation condition.
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