期刊
INTERNATIONAL IMMUNOLOGY
卷 17, 期 3, 页码 269-278出版社
OXFORD UNIV PRESS
DOI: 10.1093/intimm/dxh206
关键词
CD4/CD8 T cell; co-stimulation; cytokine; ICOS
类别
While the ligand of inducible co-stimulator (ICOS), B7 homologus protein, is widely expressed in somatic cells, B7-1 and B7-2 expression is mainly limited to lymphoid organs. Thus, the activation of T cells through ICOS without a CD28-mediated signal may occur in physiological situations. In order to gain a better understanding of the role of the ICOS co-stimulatory signal in immune responses, we studied the cellular response of T cells to beads coated with anti-ICOS or anti-CD28, plus sub-optimal anti-CD3 mAb. We demonstrate that while CD28 ligation induced expansion of both CD4(+) and CD8(+) populations, ICOS ligation only resulted in the expansion of CD8(+) T cells, and induced apoptosis in the CD4(+) T cell population. It was found that IL-2 is critically required for CD8(+) T cell expansion triggered by ICOS ligation, whereas it had only a limited effect on the expansion of CD4(+) T cells. This distinct reactivity of CD4(+) and CD8(+) T cell populations to exogenous IL-2 strongly correlates with the expression level of IL-2 receptor beta-chain, CD122, on T cells. Furthermore, we defined a small but distinct population of memory phenotype CD4(+) T cells that constitutively express ICOS. Interestingly, while naive CD4(+) T cells were unable to produce IL-2, ICOS-expressing T cells produced a substantial amount of IL-2 by stimulation with anti-ICOS/CD3 beads, suggesting that IL-2, which is indispensable for CD8(+) T cell expansion, is produced by this ICOS-expressing T cell population. These results provide evidence indicating that the ICOS co-stimulatory signal plays a distinct role in the development of CD4(+) and CD8(+) T cell-mediated immune responses.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据