期刊
EXPERIMENTAL GERONTOLOGY
卷 40, 期 3, 页码 181-188出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.exger.2004.11.002
关键词
aging; DNA oxidation; 8-hydroxy-deoxyguanosine; caloric restriction; immunohistochemistry
8-Hydroxy-deoxyguanosine adducts (8-OHdG), indices of oxidative DNA damage, were measured by immunohystochemistry with diaminobenzidine detection in the brain, skeletal muscle, heart, liver, tenuum mucosa and lymphocytes from young (4 months) and aged (24 months) Sprague-Dawley rats fed ad libitum or held on two different caloric restriction diets (alternate day ad libitum feeding or daily feeding with 40% reduced calories). In the absence of caloric restriction the levels of oxidative DNA damage increased as a function of age in all tissues examined, with a maximum similar to 3-fold increase being detected in the peripheral lymphocytes and the heart and a minimum similar to 2-fold increase being detected in the liver and brain tissues. Caloric restriction regimens effectively reduced age-dependent increase of oxidative DNA damage in all tissues examined; in particular, the brain and small intestine did not exhibit any age-related increase of oxidative DNA damage. We propose that the levels of 8-OHdG in peripheral lymphocytes may serve a biochemical index of age-related whole organism oxidative DNA damage. Immunohistochemistry might be exploited as a rapid and simple techniques for measuring lymphocytes oxidative DNA damage in large scale studies. (c) 2004 Elsevier Inc. All rights reserved.
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