期刊
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
卷 97, 期 16, 页码 7195-7204出版社
SPRINGER
DOI: 10.1007/s00253-013-5020-9
关键词
Flavonoid biosynthesis; Metabolic engineering; O-Methyltransferase; Ponciretin; Sakuranetin
资金
- Next-Generation BioGreen 21 Program [PJ00948301]
- Rural Development Administration, Republic of Korea
- Priority Research Centers Program through the National Research Foundation of Korea
- Ministry of Education, Science and Technology [2012-0006686]
Two bioactive O-methylflavonoids, sakuranetin (7-O-methylnaringenin) and ponciretin (7-O-methylnaringenin), were synthesized in Escherichia coli. Sakuranetin inhibits germination of Magnaporthe grisea, and ponciretin is a potential inhibitor of Helicobacter pylori. To achieve this, we reconstructed the naringenin biosynthesis pathway in E. coli. First, the shikimic acid pathway, which leads to the biosynthesis of tyrosine, was engineered in E. coli to increase the amount of available tyrosine. Second, several genes for the biosynthesis of ponciretin and sakuranetin such as tyrosine ammonia lyase (TAL), 4-coumaroyl CoA ligase (4CL), chalcone synthase (CHS), and O-methyltransferase (OMT) were overexpressed. In order to increase the supply the Coenzyme A (CoA), one gene (icdA, isocitrate dehydrogenase) was deleted. Using these strategies, we synthesized ponciretin and sakuranetin from glucose in E. coli at the concentration of 42.5 mg/L and 40.1 mg/L, respectively.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据