期刊
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
卷 97, 期 19, 页码 8591-8598出版社
SPRINGER
DOI: 10.1007/s00253-012-4673-0
关键词
Atomic force microscopy; Biodegradable; Cutinase; Polyesters; Surface plasmon resonance
资金
- Ministry of the Environment, KAKENHI [235658083]
- MEXT KAKENHI [23106722]
- National Institute for Agro-Environmental Sciences, Japan
- Grants-in-Aid for Scientific Research [23658083] Funding Source: KAKEN
Enzymatic degradation of polyester films by a cutinase-like enzyme from Pseudozyma antarctica JCM10317 (PaE) was analyzed by surface plasmon resonance (SPR). The adsorption of PaE and the degradation rate for polyester films were quantitatively monitored by a positive and negative SPR signal shifts, respectively. The decrease in SPR signal and the erosion depth of amorphous poly(l-lactide) (a-PLLA) film measured by atomic force microscopy (AFM) had a linear relationship, and the weight loss was estimated from the AFM data combined with a density of a-PLLA film. Furthermore, SPR sensorgrams for various polyester films showed that degradation rate of poly(epsilon-caprolactone) and poly(butylene succinate-co-adipate) which contain C6 units was higher than that of other polyesters such as poly(butylene succinate) and a-PLLA. These results suggest that C6 is the preferred chain length as substrates for PaE.
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