期刊
PROCESS BIOCHEMISTRY
卷 40, 期 3-4, 页码 1469-1474出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.procbio.2004.06.030
关键词
wheat germ; glycoprotein; water-soluble extracts; SDS-PAGE; beta-elimination
A novel glycoprotein, referred to as WGGP, was isolated from wheat germ water-soluble extracts, and purified by ion-exchange and gel filtration column chromatography. The homogeneity of WGGP was demonstrated by gel filtration on Sepharose CL-6B. The galycoprotein contains 56.4% of protein, rich in glutamic acid, asparagic acid, alanine, glycine, valine and leucine. Mannose accounted for 94% of the neutral sugar moiety, and traces of arabinose, xylose, glucose and galactose were found in WGGP. The molecular weight was estimated to be about 40,000 on SDS-PAGE. The existence of O-glycosidic linkage in WGGP was demonstrated with a beta-elimination reaction. (C) 2004 Elsevier Ltd. All rights reserved.
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