期刊
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
卷 97, 期 3, 页码 1121-1130出版社
SPRINGER
DOI: 10.1007/s00253-012-3978-3
关键词
alpha-L-Arabinofuranosidase; Glycoside hydrolase family 51; Glycoside hydrolase family 54; Expression; Penicillium chrysogenum
资金
- Japan Society for the Promotion of Science [22580091]
- Grants-in-Aid for Scientific Research [22580091] Funding Source: KAKEN
We previously isolated two alpha-l-arabinofuranosidases (ABFs), termed AFQ1 and AFS1, from the culture filtrate of Penicillium chrysogenum 31B. afq1 and afs1 complementary DNAs encoding AFQ1 and AFS1 were isolated by in vitro cloning. The deduced amino acid sequences of AFQ1 and AFS1 are highly similar to those of Penicillium purpurogenum ABF 2 and ABF 1, respectively, which belong to glycoside hydrolase (GH) families 51 and 54, respectively. Pfam analysis revealed an Alpha-L-AF_C domain in AFQ1 and ArabFuran-catal and AbfB domains in AFS1. Semi-quantitative RT-PCR analysis indicated that the afq1 gene was constitutively expressed in P. chrysogenum 31B at a low level, although the expression was slightly induced with arabinose, arabinitol, arabinan, and arabinoxylan. In contrast, expression of the afs1 gene was strongly expressed by the above four carbohydrates and less strongly induced by galactan. Recombinant enzymes (rAFQ1 and rAFS1) expressed in Escherichia coli were active against both p-nitrophenyl alpha-l-arabinofuranoside and polysaccharides with different specificities. H-1-NMR analysis revealed that rAFS1 degraded arabinofuranosyl side chains that were both singly and doubly linked to the backbones of arabinoxylan and l-arabinan. On the other hand, rAFQ1 preferentially released arabinose linked to C-3 of single-substituted xylose or arabinose residues in the two polysaccharides.
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