期刊
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
卷 96, 期 1, 页码 171-181出版社
SPRINGER
DOI: 10.1007/s00253-012-4087-z
关键词
Lactic acid bacteria; mCherry; GFP; Divergent promoters; Expression vectors
资金
- Spanish Ministry of Science and Innovation [AGL2009-12998-C03-01, AGL2009-13361-C02-02, CSD2007-00063]
- Comunidad de Madrid [ALIBIRD P2009/AGR-1469]
- European Union [238490]
Fluorescent reporter genes are valuable tools for real-time monitoring of gene expression in living cells. In this study we describe the construction of novel promoter-probe vectors containing a synthetic mCherry fluorescent protein gene, codon-optimized for lactic acid bacteria, divergently linked, or not, to a gene encoding the S65T and F64L variant of the green fluorescent protein. The utility of the transcriptional fusion vectors was demonstrated by the cloning of a single or two divergent promoter regions and by the quantitative evaluation of fluorescence during growth of Lactococcus lactis, Enterococcus faecalis, and Escherichia coli.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据