期刊
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
卷 97, 期 3, 页码 1081-1091出版社
SPRINGER
DOI: 10.1007/s00253-012-4381-9
关键词
Denaturation constant; Enthalpy; Immobilization; SiO2; Xylanase; Xylooligosaccharide
资金
- National Research Foundation of Korea
- Ministry of Education, Science and Technology [2011-50210]
- Brain Pool of Konkuk University, Republic of Korea
Enhanced catalytic activities of different lignocellulases were obtained from Armillaria gemina under statistically optimized parameters using a jar fermenter. This strain showed maximum xylanase, endoglucanase, cellobiohydrolase, and beta-glucosidase activities of 1,270, 146, 34, and 15 U mL(-1), respectively. Purified A. gemina xylanase (AgXyl) has the highest catalytic efficiency (k (cat)/K (m) = 1,440 mg mL(-1) s(-1)) ever reported for any fungal xylanase, highlighting the significance of the current study. We covalently immobilized the crude xylanase preparation onto functionalized silicon oxide nanoparticles, achieving 117 % immobilization efficiency. Further immobilization caused a shift in the optimal pH and temperature, along with a fourfold improvement in the half-life of crude AgXyl. Immobilized AgXyl gave 37.8 % higher production of xylooligosaccharides compared to free enzyme. After 17 cycles, the immobilized enzyme retained 92 % of the original activity, demonstrating its potential for the synthesis of xylooligosaccharides in industrial applications.
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