期刊
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
卷 95, 期 5, 页码 1135-1154出版社
SPRINGER
DOI: 10.1007/s00253-012-4262-2
关键词
Rumen bacteria; Archaea; Anaerobic fungi; Ribosomal RNA; mcrA; DNA fingerprinting; Microbial diversity
资金
- Indian Council of Agriculture Research (ICAR), Government of India
Rumen microbial community comprising of bacteria, archaea, fungi, and protozoa is characterized not only by the high population density but also by the remarkable diversity and the most complex microecological interactions existing in the biological world. This unprecedented biodiversity is quite far from full elucidation as only about 15-20 % of the rumen microbes are identified and characterized till date using conventional culturing and microscopy. However, the last two decades have witnessed a paradigm shift from cumbersome and time-consuming classical methods to nucleic acid-based molecular approaches for deciphering the rumen microbial community. These techniques are rapid, reproducible and allow both the qualitative and quantitative assessment of microbial diversity. This review describes the different molecular methods and their applications in elucidating the rumen microbial community.
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