期刊
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
卷 96, 期 5, 页码 1291-1300出版社
SPRINGER
DOI: 10.1007/s00253-012-4177-y
关键词
Escherichia coli; Metabolic engineering; Metabolic evolution; Fuel ethanol; Glucose; Acetate
资金
- Mexican Council of Science and Technology (CONACyT) [Proinnova PETRA-MIN 2011/154298-2012/181892, FONSEC/SSA 126793, IMSS/ISSSTE 167756, DGAPA/PAPIIT/UNAM IN221106, IT200312-2]
- CONACyT
In this work, Escherichia coli MG1655 was engineered to produce ethanol and evolved in a laboratory process to obtain an acetate tolerant strain called MS04 (E. coli MG1655: Delta pflB, Delta adhE, Delta frdA, Delta xylFGH, Delta ldhA, PpflB::pdc (Zm) -adhB (Zm) , evolved). The growth and ethanol production kinetics of strain MS04 were determined in mineral medium, mainly under non-aerated conditions, supplemented with glucose in the presence of different concentrations of sodium acetate at pH 7.0 and at different values of acid pH and a constant concentration of sodium acetate (2 g/l). Results revealed an increase in the specific growth rate, cell mass formation, and ethanol volumetric productivity at moderate concentrations of sodium acetate (2-10 g/l), in addition to a high tolerance to acetate because it was able to grow and produce a high yield of ethanol in the presence of up to 40 g/l of sodium acetate. Genomic analysis of the Delta pflB evolved strain identified that a chromosomal deletion of 27.3 kb generates the improved growth and acetate tolerance in MG1655 Delta pflB derivative strains. This deletion comprises genes related to the respiration of nitrate, repair of alkylated DNA and synthesis of the ompC gene coding for porin C, cytochromes C, thiamine, and colonic acid. Strain MS04 is advantageous for the production of ethanol from hemicellulosic hydrolysates that contain acetate.
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