期刊
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
卷 95, 期 3, 页码 635-645出版社
SPRINGER
DOI: 10.1007/s00253-012-4064-6
关键词
Asymmetric reduction; Old yellow enzyme; Enoate reductase; Activated alkene; Achromobacter sp.; Baclofen
资金
- National Natural Science Foundation of China [20802073, 21072183]
- 100 Talents Program
- West Light Foundation of the Chinese Academy of Sciences
The strain Achromobacter sp. JA81, which produced enoate reductase, was applied in the asymmetric reduction of activated alkenes. The strain could catalyze the bioreduction of alkenes to form enantiopure (R)-beta-aryl-beta-cyano-propanoic acids, a precursor of (R)-gamma-amino butyric acids, including the pharmaceutically active enantiomer of the chiral drug (R)-baclofen with excellent enantioselectivity. It could catalyze as well the stereoselective bioreduction of other activated alkenes such as cyclic imides, beta-nitro acrylates, and nitro-alkenes with up to > 99 % ee and > 99 % conversion. The draft genome sequencing of JA81 revealed six putative old yellow enzyme homologies, and the transcription of one of them, Achr-OYE3, was detected using reverse transcription polymerase chain reaction. The recombinant Escherichia coli expressing Achr-OYE3 displayed enoate reductase activity toward (Z)-3-cyano-3-phenyl-propenoic acid (2a).
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