期刊
JOURNAL OF CHROMATOGRAPHY A
卷 1067, 期 1-2, 页码 303-309出版社
ELSEVIER
DOI: 10.1016/j.chroma.2004.12.063
关键词
meat; food analysis; growth hormones; freezing-lipid filtration; SPE clean-up; GC-MS
Estradiol, testosterone, progesterone, zeranol and diethylstilbestrol including estradiol metabolites were determined simultaneously in meat. Extraction of growth hormones was carried out by ultasonication using a methanol-water mixture. The growth hormones in the meat extract can be effectively separated from lipids by freezing-lipid filtration, followed by C-8-solid phase extraction (SPE). During freezing-lipid filtration, about 90% of lipids are removed without any significant loss of growth hormones. For further clean-up, silica- and aminopropyl-SPE were used. To enhance detection sensitivity, the growth hormones are derivatized with trimethylsilyl reagents. Quantitation using isotope-labelled internal standards was performed by gas chromatography-mass spectrometry in the selected ion monitoring mode. The method detection limits were 0.1-0.4 mu g/kg for all growth hormones. Overall recoveries of synthetic and natural growth hormones were 68-106% with coefficients of variation of 5-16% for the complete procedure. (c) 2004 Published by Elsevier B.V.
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