期刊
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
卷 91, 期 6, 页码 1623-1632出版社
SPRINGER
DOI: 10.1007/s00253-011-3327-y
关键词
Wickerhamiella domercqiae var. sophorolipid CGMCC 1576; Lactonic sophorolipid; Acidic sophorolipid; Organic nitrogen source; Inorganic nitrogen source
资金
- Encouragement Foundation of Shandong Province for Young Scientists [2006BS02010]
- Key Scientific and Technological project of Shandong Province [2007GG10002002]
- National Natural Science Foundation of China [30870048, 30970052]
- National Key Technology R&D Program in the 11th Five-Year Plan of China [2008BAI63B08]
The effects of nitrogen sources on growth of sophorolipid-producing yeast, Wickerhamiella domercqiae var. sophorolipid CGMCC 1576 and on production and composition of sophorolipids were studied. Organic nitrogen sources are more favorable for accumulation of biomass than inorganic ones. Presence of ammonium ion from different inorganic nitrogen sources (except NH(4)HCO(3)) greatly inhibited the production of lactonic sophorolipids. However, when organic nitrogen sources were used, lactonic sophorolipid production was strongly increased. Production of crystalline lactonic sophorolipids from organic/inorganic nitrogen sources was enhanced with the increase of pH value adjusted by sodium hydroxide or sodium citrate solution. Fourier-transform infrared (FT-IR), gas chromatography mass spectrometry (GC-MS), high-performance liquid chromatography (HPLC), and mass spectra (MS) were employed to compare the composition of sophorolipid mixture obtained from different nitrogen sources. More than 15 acidic sophorolipid molecules and only 4 lactonic sophorolipid molecules were produced by using 1.27 g/l ammonium sulfate as nitrogen source; they were separated by preparative HPLC and their structures were elucidated by MS. These results suggest extraordinary regulatory effects of nitrogen source on growth and sophorolipid synthesis of W. domercqiae var. sophorolipid.
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