期刊
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
卷 91, 期 4, 页码 1203-1213出版社
SPRINGER
DOI: 10.1007/s00253-011-3407-z
关键词
Escherichia coli; Recombinant protein production; Autoinduction; Defined medium
资金
- FORSYS
Protein production under the control of lac operon regulatory elements using autoinduction is based on diauxic growth of Escherichia coli on lactose after consumption of more preferred carbon substrates. A novel simple and cost-effective defined autoinduction medium using a mixture of glucose, glycerol, and lactose as carbon substrate and NH (4) (+) as sole nitrogen source without any supplementation of amino acids and vitamins was developed for T7-based E. coli expression systems. This medium was successfully employed in 96-well microtiter plates, test tubes, shake flasks, and 15-L bioreactor cultivations for production of different types of proteins achieving an average yield of 500 mg L-1 product. Cell-specific protein concentrations and solubility were similar as during conventional isopropyl beta-d-1-thiogalactopyranoside induction using Luria-Bertani broth. However, the final yield of target proteins was about four times higher, as a higher final biomass was achieved using this novel defined autoinduction broth.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据