期刊
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
卷 90, 期 3, 页码 895-901出版社
SPRINGER
DOI: 10.1007/s00253-011-3116-7
关键词
Corynebacterium glutamicum; Endoglucanase; Signal sequence; Glutamate fermentation; beta-Glucan
资金
- Ministry of Education, Culture, Sports, Science and Technology of Japan
- JSPS [20859]
We demonstrate glutamate production from beta-glucan using endoglucanase (EG)-expressing Corynebacterium glutamicum. The signal sequence torA derived from Escherichia coli K12, which belongs to the Tat pathway, was suitable for secreting EG of Clostridium thermocellum using C. glutamicum as a host. Using the torA signal sequence, endoglucanase from Clostridium cellulovorans 743B was successfully expressed, and the secreted EG produced 123 mg of reducing sugar from 5 g of beta-glucan at 30 A degrees C for 72 h, which is the optimal condition for C. glutamicum growth. Subsequently, glutamate fermentation from beta-glucan was carried out with the addition of Aspergillus aculeatus beta-glucosidase produced by recombinant Aspergillus oryzae. Using EG-secreting C. glutamicum, 178 mg/l of glutamate was produced from 15 g of beta-glucan. This is the first report of glutamate fermentation from beta-glucan using endoglucanase-secreting C. glutamicum.
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