期刊
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
卷 92, 期 6, 页码 1179-1185出版社
SPRINGER
DOI: 10.1007/s00253-011-3396-y
关键词
Bifidobacterium adolescentis; Xylooligosaccharides; Prebiotic; Betaxylosidase
资金
- Instituut voor de aanmoediging van Innovatie door Wetenschap en Technologie in Vlaanderen (SBO IMPAXOS)
- Research Fund K.U. Leuven [IDO/03/005]
Xylooligosaccharides have strong bifidogenic properties and are increasingly used as a prebiotic. Nonetheless, little is known about the degradation of these substrates by bifidobacteria. We characterized two recombinant beta-xylosidases, XylB and XylC, with different substrate specificities from Bifidobacterium adolescentis. XylB is a novel beta-xylosidase that belongs to the recently introduced glycoside hydrolase family 120. In contrast to most reported beta-xylosidases, it shows only weak activity on xylobiose and prefers xylooligosaccharides with a degree of polymerization above two. The remaining xylobiose is efficiently hydrolyzed by the second B. adolescentis beta-xylosidase, XylC, a glycoside hydrolase of family 43. Furthermore, XylB releases more xylose from arabinose-substituted xylooligosaccharides than XylC (30% and 20%, respectively). The different specificities of XylB, XylC, and the recently described reducing-end xylose-releasing exo-oligoxylanase RexA show how B. adolescentis can efficiently degrade prebiotic xylooligosaccharides.
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