期刊
JOURNAL OF EXPERIMENTAL MEDICINE
卷 201, 期 5, 页码 681-686出版社
ROCKEFELLER UNIV PRESS
DOI: 10.1084/jem.20041959
关键词
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The antigenic targets recognized by naturally occurring CD4(+)CD25(+) regulatory T cells (T reg cells) have been elusive. We have serologically defined a series of broadly expressed self-antigens derived from chemically induced mouse sarcomas by serological identification of antigens by recombinant expression cloning (SEREX). CD4(+)CD25(+)T cells from mice immunized with SEREX-defined self-antigens had strong suppressive activity on peptide-specific proliferation of CD4(+)CD25(-)T cells and CD8(+)T cells. The suppressive effect was observed without in vitro T cell stimulation. Foxp3 expression in these CD4(+)CD25(+)T cells from immunized mice was 5-10 times greater than CD4(+)CD25(+)T cells derived from naive mice. The suppressive effect required cellular contact and was blocked by anti-glucocorticoid-induced tumor necrosis factor receptor family-related gene antibody. In vitro suppressive activity essentially disappeared 8 wk after the last immunization. However, it was regained by in vitro restimulation with cognate self-antigen protein but not with control protein. We propose that SEREX-defined self-antigens such as those used in this study represent self-antigens that elicit naturally occurring CD4(+)CD25(+) T reg cells.
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