期刊
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
卷 95, 期 2, 页码 511-520出版社
SPRINGER
DOI: 10.1007/s00253-011-3696-2
关键词
2-Fluorophenol (2-FP); Rhodococcus sp.; Biodegradation; Luong model; Monooxygenase
资金
- Fundacao para a Ciencia e Tecnologia (FCT), Portugal [SFRH/BD/30771/2006, SFRH/BPD/44670/2008]
- Fundo Social Europeu (FSE) (Programa Operacional Potencial Humano (POPH), Quadro de Referencia Estrategico Nacional (QREN)
- FCT [POCI/V.5/A0105/2005, PTDC/BIO/67306/2006]
- Higher Education Commission (HEC), Government of Pakistan
- Fundação para a Ciência e a Tecnologia [SFRH/BPD/44670/2008, POCI/V.5/A0105/2005, PTDC/BIO/67306/2006, SFRH/BD/30771/2006] Funding Source: FCT
A pure bacterial culture able to utilize 2-fluorophenol (2-FP) as sole carbon and energy source was isolated by selective enrichment from sediments collected from a contaminated site in Northern Portugal. 16S rRNA gene analysis showed that the organism (strain FP1) belongs to the genus Rhodococcus. When grown aerobically on 2-FP, growth kinetics of strain FP1 followed the Luong model. An inhibitory effect of increasing 2-FP concentrations was observed with no growth occurring at 2-FP levels higher than ca. 4 mM. Rhodococcus strain FP1 was able to degrade a range of other organofluorine compounds, including 2-fluorobenzoate, 3-fluorobenzoate, 4-fluorobenzoate, 3-fluorophenol, 4-fluorophenol, 3-fluorocatechol, and 4-fluorocatechol, as well as chlorinated compounds such as 2-chlorophenol and 4-chlorophenol. Experiments with cell-free extracts and partially purified enzymes indicated that the first step of 2-fluorophenol metabolism was conversion to 3-fluorocatechol, suggesting an unusual pathway for fluoroaromatic metabolism. To our knowledge, this is the first time that utilization of 2-FP as a growth substrate by a pure bacterial culture is reported.
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