期刊
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
卷 90, 期 4, 页码 1323-1332出版社
SPRINGER
DOI: 10.1007/s00253-011-3157-y
关键词
alpha-Glucuronidase; Schizophyllum commune; GH115; Gene expression; Xylan degradation
资金
- Academy of Finland [1124281]
- Glycoscience Graduate School
- Short Term Scientific Mission (STSM) [COST 928, COST FP0602]
- Dutch Technology Foundation STW
- science division of NWO
- Ministry of Economic Affairs [07063]
Schizophyllum commune produces an alpha-glucuronidase that is active on polymeric xylan, while the ascomycete alpha-glucuronidases are only active on xylan oligomers. In this study, we have identified the gene (agu1) encoding this enzyme and confirmed the functionality by overexpression of the gene in S. commune and degradation of aldopentauronic acids, (MeGlcA)(3)-Xyl(4), in the cultivation medium of the transformants. Expression analysis demonstrated that agu1 is not co-regulated with the predominant xylanase-encoding gene (xynA) of S. commune. The detailed sequence analysis of Agu1 demonstrated that this gene belongs to a novel glycoside hydrolase family (GH115) that also contains candidate genes from ascomycete fungi and bacteria. Phylogenetic analysis showed that the fungal GH115 alpha-glucuronidases are distinctly separate from the prokaryotic clade and distributed over three branches. The identification of putative genes encoding this enzyme in industrial fungi, such as Aspergillus oryzae and Hypocrea jecorina, will provide a starting point for further analysis of the importance of this enzyme for the hydrolysis of plant biomass.
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