期刊
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
卷 53, 期 5, 页码 1782-1790出版社
AMER CHEMICAL SOC
DOI: 10.1021/jf0484982
关键词
neohesperidin dihydrochalcone; hesperetin dihydrochalcone 4 '-beta-D-glucoside; hesperetin dihydrochalcone; 3-(3-hydroxy-4-methoxyphenyl)propionic acid; 3-(3,4-dihydroxyphenyl)propionic acid; Eubacterium ramulus; Clostridium orbiscindens; phloretin hydrolase
The degradation of neohesperidin dihydrochalcone by human intestinal microbiota was studied in vitro. Human fecal slurries converted neohesperidin dihydrochalcone anoxically to 3-(3-hydroxy-4-methoxyphenyl)propionic acid or 3-(3,4-dihydroxyphenyl)propionic acid. Two transient intermediates were identified as hesperetin dihydrochalcone 4'-beta-D-glucoside and hesperetin dihydrochalcone. These metabolites suggest that neohesperdin dihydrochalcone is first deglycosylated to hesperetin dihydrochalcone 4'-beta-D-glucoside and subsequently to the aglycon hesperetin dihydrochalcone, The latter is hydrolyzed to the corresponding 3-(3-hydroxy-4-methoxyphenyl)propionic acid and probably phloroglucinol. Eubacterium ramulus and Clostridium orbiscindens were not capable of converting neohesperidin dihydrochalcone. However, hesperetin dihydrochalcone 4'-beta-D-glucoside was converted by E. ramulus to hesperetin dihydrochalcone and further to 3-(3-hydroxy-4-methoxyphenyl)propionic acid, but not by C. orbiscindens. In contrast, hesperetin dihydrochalcone was cleaved to 3-(3-hydroxy-4-methoxyphenyl)propionic acid by both species. The latter reaction was shown to be catalyzed by the phloretin hydrolase from E. ramulus.
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