期刊
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
卷 89, 期 1, 页码 145-155出版社
SPRINGER
DOI: 10.1007/s00253-010-2953-0
关键词
Xylanosome; Penicillium purpurogenum; Sugar beet pulp; Hemicellulose degradation; Multienzyme complexes; Secretome
资金
- FONDECYT [1070368, 1100084]
- UNAB [DI-07-06/I]
- NIH, National Center for Research Resources [2 P20 RR016479]
- NATIONAL CENTER FOR RESEARCH RESOURCES [P20RR016479] Funding Source: NIH RePORTER
Proteins secreted by filamentous fungi play key roles in different aspects of their biology. The fungus Penicillium purpurogenum, used as a model organism, is able to degrade hemicelluloses and pectins by secreting a variety of enzymes to the culture medium. This work shows that these enzymes interact with each other to form high molecular weight, catalytically active complexes. By using a proteomics approach, we were able to identify several protein complexes in the secretome of this fungus. The expression and assembly of these complexes depend on the carbon source used and display molecular masses ranging from 300 to 700 kDa. These complexes are composed of a variety of enzymes, including arabinofuranosidases, acetyl xylan esterases, feruloyl esterases, beta-glucosidases and xylanases. The protein-protein interactions in these multienzyme complexes were confirmed by coimmunoprecipitation assays. One of the complexes was purified from sugar beet pulp cultures and the subunits identified by tandem mass spectrometry. A better understanding of the biological significance of these kinds of interactions will help in the comprehension of the degradation mechanisms used by fungi and may be of special interest to the biotechnology industry.
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