期刊
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
卷 89, 期 6, 页码 2019-2025出版社
SPRINGER
DOI: 10.1007/s00253-010-2952-1
关键词
Petroleum hydrocarbons; beta-cyclodextrin; Electrokinetic remediation; Denaturing gradient gel electrophoresis (DGGE); Bacterial community
资金
- National High Technology Research and Development Program (863 Program) of China [2007AA061200]
- Educational Commission of Heilongjiang province of China [11544038]
- National Important Science and Technology Water Projects of China [2008ZX07211-007]
Electrokinetic (EK) migration of beta-cyclodextrin (beta-CD), which is inclusive of total petroleum hydrocarbon (TPH), is an economically beneficial and environmentally friendly remediation process for oil-contaminated soils. Remediation studies of oil-contaminated soils generally prepared samples using particular TPHs. This study investigates the removal of TPHs from, and electro-migration of microbial cells in field samples via EK remediation. Both TPH content and soil respiration declined after the EK remediation process. The strains in the original soil sample included Bacillus sp., Sporosarcina sp., Beta proteobacterium, Streptomyces sp., Pontibacter sp., Azorhizobium sp., Taxeobacter sp., and Williamsia sp. Electromigration of microbial cells reduced the biodiversity of the microbial community in soil following EK remediation. At 200 Vm(-1) for 10 days, 36% TPH was removed, with a small population of microbial cells flushed out, demonstrating that EK remediation is effective for the present oil-contaminated soils collected in field.
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