期刊
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
卷 87, 期 2, 页码 679-690出版社
SPRINGER
DOI: 10.1007/s00253-010-2626-z
关键词
Pseudomonas putida S12; p-hydroxybenzoate; Transcriptomics; Proteomics; Metabolic engineering
资金
- Netherlands Ministry of Economic Affairs
- B-Basic partner organizations
- Kluyver Centre for Genomics of Industrial Fermentation, Netherlands Genomics Initiative/Netherlands Organization for Scientific Research
A transcriptomics and proteomics approach was employed to study the expression changes associated with p-hydroxybenzoate production by the engineered Pseudomonas putida strain S12palB1. To establish p-hydroxybenzoate production, phenylalanine-tyrosine ammonia lyase (pal/tal) was introduced to connect the tyrosine biosynthetic and p-coumarate degradation pathways. In agreement with the efficient p-hydroxybenzoate production, the tyrosine biosynthetic and p-coumarate catabolic pathways were upregulated. Also many transporters were differentially expressed, one of which-a previously uncharacterized multidrug efflux transporter with locus tags PP1271-PP1273-was found to be associated with p-hydroxybenzoate export. In addition to tyrosine biosynthesis, also tyrosine degradative pathways were upregulated. Eliminating the most prominent of these resulted in a 22% p-hydroxybenzoate yield improvement. Remarkably, the upregulation of genes contributing to p-hydroxybenzoate formation was much higher in glucose than in glycerol-cultured cells.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据