期刊
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
卷 86, 期 5, 页码 1439-1449出版社
SPRINGER
DOI: 10.1007/s00253-009-2386-9
关键词
Cell-wall-degrading enzyme; Podoviridae; Bacteriophage; Biofilm; Staphylococcus aureus
资金
- Korean Ministry of Education, Science, and Technology
- Research Institute for Agriculture and Life Sciences, Seoul National University
Antibacterial and biofilm removal activity of a new podoviridae Staphylococcus aureus bacteriophage (SAP-2), which belongs to the phi 29-like phage genus of the Podoviridae family, and a cell-wall-degrading enzyme (SAL-2), which is derived from bacteriophage SAP-2, have been characterized. The cell-wall-degrading enzyme SAL-2 was expressed in Escherichia coli in a soluble form using a low-temperature culture. The cell-wall-degrading enzyme SAL-2 had specific lytic activity against S. aureus, including methicillin-resistant strains, and showed a minimum inhibitory concentration of about 1 mu g/ml. In addition, this enzyme showed a broader spectrum of activity within the Staphylococcus genus compared with bacteriophage SAP-2 in its ability to remove the S. aureus biofilms. Thus, the cell-wall-degrading enzyme SAL-2 can be used to prevent and treat biofilm-associated S. aureus infections either on its own or in combination with other cell-wall-degrading enzymes with anti-S. aureus activity.
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