期刊
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
卷 85, 期 4, 页码 1069-1079出版社
SPRINGER
DOI: 10.1007/s00253-009-2108-3
关键词
Streptomyces; Secondary metabolite; alpha-Lipomycin; Regulation
资金
- BMBF
- DAAD [PKZ A/07/99406]
Analysis of the alpha-lipomycin biosynthesis gene cluster of Streptomyces aureofaciens Tu117 led to the identification of five putative regulatory genes, which are congregated into a subcluster. Analysis of the lipReg1-4 and lipX1 showed that they encode components of two-component signal transduction systems (LipReg1 and LipReg2), multiple antibiotics resistance-type regulator (LipReg3), large ATP-binding regulators of the LuxR family-type regulator (LipReg4), and small ribonuclease (LipRegX1), respectively. A combination of targeted gene disruptions, complementation experiments, lipomycin production studies, and gene expression analysis via RT-PCR suggests that all regulatory lip genes are involved in alpha-lipomycin production. On the basis of the obtained data, we propose that LipReg2 controls the activity of LipReg1, which in its turn govern the expression of the alpha-lipomycin pathway-specific regulatory gene lipReg4. The ribonuclease gene lipX1 and the transporter regulator lipReg3 appear to work independently of genes lipReg1, lipReg2, and lipReg4.
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