期刊
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
卷 82, 期 4, 页码 713-719出版社
SPRINGER
DOI: 10.1007/s00253-008-1808-4
关键词
Cell surface engineering; SED1; leu2-d; DsRed-monomer; Display efficiency
Vector engineering and gene disruption in host cells were attempted for the enhancement of alpha-agglutinin-based display of proteins on the cell surface in yeast. To evaluate the display efficiency by flow cytometric analysis, DsRed-monomer fused with FLAG-tag was displayed and immunostained as a model protein. The use of leu2-d in the expression vector resulted in the enhanced efficiency and ratio of the accessible display of proteins. Moreover, the amount of displayed proteins in SED1-disrupted cells increased particularly during the stationary growth phase. The combination of these improvements resulted in the quantitatively enhanced accessible display of DsRed-monomer on the yeast cell surface. The improved yeast display system would be useful in a wider range of its applications in biotechnology.
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