期刊
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
卷 85, 期 6, 页码 1907-1914出版社
SPRINGER
DOI: 10.1007/s00253-009-2247-6
关键词
Bacillus subtilis; Glucose-6-phosphate dehydrogenase; Metabolic flux analysis; Riboflavin; Ribulose-5-phosphate
资金
- National Natural Science Foundation of China [NSFC20536040]
- National Project of Key Fundamental Research [2007CB707802]
- Development Project of Science and Technology of Tianjin [05YFGZGX04500]
Carbon flow in Bacillus subtilis through the pentose phosphate (PP) pathway was modulated by overexpression of glucose-6-phosphate dehydrogenase (G6PDH) under the control of the inducible Pxyl promoter in B. subtilis PY. Alteration of carbon flow into the PP pathway will affect the availability of ribulose-5-phosphate (Ru5P) and the riboflavin yield. Overexpression of G6PDH resulted in the glucose consumption rate increasing slightly, while the specific growth rate was unchanged. An improvement by 25% +/- 2 of the riboflavin production was obtained. Compared to by-products formation in flask culture, low acid production (acetate and pyruvate) and more acetoin were observed. Metabolic analysis, together with carbon flux redistribution, indicated that the PP pathway fluxes are increased in response to overexpression of G6PDH. Moreover, increased flux of the PP pathway is associated with an increased intracellular pool of Ru5P, which is a precursor for riboflavin biosynthesis. The high concentrations of Ru5P could explain the increased riboflavin production.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据