期刊
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
卷 85, 期 4, 页码 975-984出版社
SPRINGER
DOI: 10.1007/s00253-009-2083-8
关键词
Metarhizium anisopliae; Beauveria bassiana; Cell wall protein; Gene cloning; Protein expression; Functional analysis
资金
- Ministry of Science and Technology of China [2009CB118904, 2007DFA3100, 2006AA10A212]
- Zhejiang RD programs [2007C12035, 2008C02007-1, 2008C12057]
A cell wall protein, CWP10, resolved from the conidial formic acid extract of a Metarhizium anisopliae isolate, was characterized as a new 9.9-kDa protein with a 32-aa signal peptide with a central hydrophobic region (ca. 10 residues) at its N-terminus. This protein was proven neither to be hydrophobic nor glycosylated and encoded by a 363-bp, single-copy gene with three introns. CWP10 was existent in the conidial extracts of seven of 18 tested M. anisopliae isolates and much more abundant (immunogold-labeled) on conidial walls than in cytoplasm. Integrating the gene into a CWP10-absent strain of Beauveria bassiana led to excellent expression of CWP10 in aerial conidia, increasing net conidial hydrophobicity by 10.8% or adhesion to hydrophobic Teflon by 1.3-fold. However, the expressed protein had no effect on conidial tolerance to thermal and ultraviolet stresses. This is the first report on a non-hydrophobic cell-wall protein enhancing conidial hydrophobicity and adhesion of the fungal species.
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