期刊
FEBS LETTERS
卷 579, 期 7, 页码 1619-1624出版社
WILEY
DOI: 10.1016/j.febslet.2005.02.016
关键词
hammerhead ribozyme; HIV-1
资金
- NIAID NIH HHS [AI42552, AI29329] Funding Source: Medline
trans-Cleaving hammerhead ribozyme variants were generated with mimicked non-conserved internal loop motifs derived from five structurally diverse natural cis-cleaving ribozymes. Most modified trans-cleaving variants showed enhanced single-turnover cleavage rates relative to minimal counterparts that lack tertiary interactions between internal loop motifs I and II, and relative to controls with sequence changes in loop I. The trans-cleaving ribozyme derived from the positive strand of peach latent mosaic viroid had the highest observed cleavage rate, suggesting a structurally optimized motif that facilitates rapid formation of the ribozyme catalytic center in a trans-reaction. (c) 2005 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
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