期刊
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
卷 85, 期 3, 页码 517-524出版社
SPRINGER
DOI: 10.1007/s00253-009-2139-9
关键词
Nonionic surfactant; Micelle; Response surface methodology; Extractive microbial transformation L-phenylacetylcarbinol
资金
- National Natural Science Foundation of China [20676080]
- State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, China
- Shanghai Jiao Tong University, Shanghai, China
Extractive microbial transformation of L-phenylacetylcarbinol (L-PAC) in nonionic surfactant Triton X-100 micelle aqueous solution was investigated by response surface methodology. Based on the Box-Behnken design, a mathematical model was developed for the predication of mutual interactions between benzaldehyde, Triton X-100, and glucose on L-PAC production. It indicated that the negative or positive effect of nonionic surfactant strongly depended on the substrate concentration. The model predicted that the optimal concentration of benzaldehyde, Triton X-100, and glucose was 1.2 ml, 15 g, and 2.76 g per 100 ml, respectively. Under the optimal condition, the maximum L-PAC production was 27.6 mM, which was verified by a time course of extractive microbial transformation. A discrete fed-batch process for verification of cell activity was also presented.
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