期刊
FEBS LETTERS
卷 579, 期 7, 页码 1602-1606出版社
WILEY
DOI: 10.1016/j.febslet.2005.01.074
关键词
total internal reflection fluorescence microscopy; insulin exocytosis; sulfonylurea receptor; fusion; syntaxin
资金
- NIDDK NIH HHS [DK-52771, DK57671] Funding Source: Medline
To explore how the sulfonylurea receptor (SUR1) is involved in docking and fusion of insulin granules, dynamic motion of single insulin secretory granules near the plasma membrane was examined in SUR1 knock-out (Sur1KO) beta-cells by total internal reflection fluorescence microscopy. Sur1KO beta-cells exhibited a marked reduction in the number of fusion events from previously docked granules. However, the number of docked granules declined during stimulation as a consequence of the release of docked granules into the cytoplasm vs. fusion with the plasma membrane. Thus, the impaired docking and fusion results in decreased insulin exocytosis from Sur1KO beta-cells. (c) 2005 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据