期刊
BRAIN RESEARCH BULLETIN
卷 65, 期 2, 页码 111-115出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.brainresbull.2004.12.001
关键词
nitric oxide synthase; cGMP-dependent protein kinase; rat cerebellum; quantitative RT-PCR
The expression of neuronal nitric oxide synthase (nNOS) and the cGMP-dependent protein kinases cGKI and cGKII in rat cerebellum was evaluated at different developmental stages by quantitative RT-PCR and Western blotting. mRNAs coding for these proteins were detected in the cerebella of rats aged 7, 14 and 21 days. Expression levels, nevertheless, varied significantly at each of these developmental stages. While nNOS and cGKI mRNA levels steadily increased during development, cGKII mRNA showed a different behaviour pattern, with similar levels observed on postnatal days 7 and 14 and increased levels noted on postnatal day 21. Moreover, protein expression profiles for nNOS and cGKI showed similar patterns to the mRNAs encoding these proteins. Our results reveal the developmental regulation of the expression of these proteins in the cerebellum, giving rise to higher levels as the cerebellum matures. (c) 2004 Elsevier Inc. All rights reserved.
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