期刊
BIOSENSORS & BIOELECTRONICS
卷 20, 期 9, 页码 1847-1850出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2004.05.010
关键词
protein chip; surface plasmon resonance; protein G; pathogen; self-assembly technique
Protein chip based on surface plasmon resonance (SPR) was developed for detection of pathogens existing in contaminated environment, such as Escherichia coli O157:H7, Salmonella typhimurium, Legionella pneumophila, and Yersinia enterocolitica. Protein G was immobilized to endow the orientation of antibody molecules on the SPR surface. The pathogen binding of the protein chip was investigated by SPR spectroscopy. Consequently, it was found that the four kinds of pathogen could be selectively detected by using SPR-based protein chip. (C) 2004 Elsevier B.V. All rights reserved.
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