期刊
JOURNAL OF PHYSIOLOGY-LONDON
卷 563, 期 3, 页码 777-793出版社
BLACKWELL PUBLISHING
DOI: 10.1113/jphysiol.2004.080655
关键词
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资金
- NIMH NIH HHS [R01 MH051290] Funding Source: Medline
To investigate the effects of persistent elevation of synaptic glycine at Schaffer collateral-CA1 synapses of the hippocampus, we studied the glutamatergic synaptic transmission in acute brain slices from mice with reduced expression of glycine transporter type 1 (GlyT1+/-) as compared to wild type (WT) littermates using whole-cell patch-clamp recordings of CA1 pyramidal cells. We observed faster decay kinetics, reduced ifenprodil sensitivity and increased zinc-induced antagonism in N-methyl-D-aspartate receptor (NMDAR) currents of GlyT1+/- mice. Moreover, the ratio ce-amino-3-hydroxy-5-methylisoxazole-4-propionic acid receptor (AMPAR)/NMDAR was decreased in mutants compared to WT. Surprisingly, this change was associated with a reduction in the number of AMPARs expressed at the CA1 synapses in the mutants compared to WT. Overall, these findings highlight the importance of GlyT1 in regulating glutamatergic neurotransmission.
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