期刊
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS
卷 15, 期 6, 页码 1725-1729出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.bmcl.2005.01.030
关键词
thiazole orange; optical fiber; biosensor; DNA; hybridization
Thiazole orange dyes were derivatized with ethylene glycol linkers of various lengths, and were covalently linked to the 5' end of the oligonucleotides after solid-phase synthesis. The labeled oligonucleotides exhibited enhanced fluorescence upon hybridization to complementary DNA sequences at the surfaces of optical fibers, providing for a self-contained labeling strategy. It was determined that the melt temperatures of DNA hybrids using one mixed polypyrimidine base oligonucleotide sequence were dependent on the length of the tethers, and that the melt temperature could be shifted by more than 10 degrees C when tethers were introduced. (c) 2005 Elsevier Ltd. All rights reserved.
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