期刊
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
卷 79, 期 1, 页码 119-126出版社
SPRINGER
DOI: 10.1007/s00253-008-1406-5
关键词
quorum sensing inhibitor; virtual screening; baicalein; peudomonus aeruginosa; biofilm; Traditional Chinese Medicine
The automated docking program DOCK 5.3.0 was applied to screening for quorum sensing inhibitors (QSIs) of Peudomonus aeruginosa from a database containing 51 active components of Traditional Chinese Medicines with antibacterial activity. Five potential QSIs were revealed by the computer-based virtual screening. The compounds 3, 4, 5, 6, 7 inhibit biofilm formation of P. aeruginosa at a concentration of 200 mu M. Compound 4 (baicalein) does not inhibit the growth of P. aeruginosa; however, it significantly inhibits biofilm formation of the bacteria at a lower concentration of 20 mu M and promoted proteolysis of the signal receptor TraR protein in Escherichia coli at 4-40 mM. Baicalein and ampicillin showed synergistic activity against P. aeruginosa. These results suggested that baicalein can interfere with quorum sensing system of P. aeruginosa and will be developed as antibacterial agent with novel target.
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