期刊
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
卷 78, 期 5, 页码 881-888出版社
SPRINGER
DOI: 10.1007/s00253-008-1365-x
关键词
polycyclic aromatic hydrocarbons; phenanthrene; Mycobacterium; biodegradation; biphasic culture; design of experiments
In the present paper, the degradation of phenanthrene, a model polycyclic aromatic hydrocarbon compound, by the Mycobacterium strain 6PY1 was optimized in a biphasic culture medium. The optimization and modeling were performed using the design of experiments methodology. The temperature, the silicone oil/mineral salts medium volume ratio, and the initial cell concentration, were used as the central composite design parameters. In all experiments, the phenanthrene was degraded to undetectable levels. Response surface methodology was successfully employed to derive an empirical model describing the rate and time of degradation and to deduce the optimal degradation conditions. As a result of the optimization processes, the optimal responses for the degradation rate, the volumetric degradation rate, and the 90% degradation time were estimated to be 0.172 mg h(-1) stop, 22 mg l(-1) h(-1), and 18 h, respectively.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据