Protein adsorption on the mesoporous molecular sieve silicate SBA-15: effects of pH and pore size

A mesoporous molecular sieve silicate, SBA- 15, with three pore sizes (38.1 angstrom, 77.3 angstrom, and 240 angstrom) has been synthesized using a non-ionic, tri-block copolymer as a template in a sol-gel method. The effects of synthesis conditions on the pore size and pore-size distribution of this adsorbent have been described. The adsorption of proteins on these crystalline, ordered, materials has been studied. The kinetics of adsorption and equilibrium capacity have been probed with three proteins of different dimensions. The effects of electrostatic interactions and protein size are illustrated. It has been shown that SBA-15 materials can be tailored to show size selectivity for proteins, and very high capacities (450 mg/g) can be obtained. Furthermore, the rates of adsorption are shown to be dependent on the pore size, protein structure and solution pH. (c) 2004 Elsevier B.V. All rights reserved.