期刊
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
卷 328, 期 4, 页码 831-837出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.bbrc.2005.01.033
关键词
parathyroid hormone; nuclear factor Y; 1,25-dihydroxyvitamin D-3; vitamin D receptor; vitamin D response element
The negative regulation of the human parathyroid hormone (PTH) gene by biologically active vitamin D-3 (1,25-dillydroxyvitainin D-3; 1,25(OH)(2)D-3) was studied in rat pituitary GH4Cl cells, which express factors needed for the negative regulation. We report here that NF-Y binds to sequences downstream of the site previously reported to bind the vitamin D receptor (VDR). Additional binding sites for NF-Y reside in the near vicinity and were shown to be important for full activity of the PTH gene promoter. VDR and NF-Y were shown to exhibit mutually exclusive binding to the VDRE region. According to our results, sequestration of binding partners for NF-Y by VDR also affects transcription through a NF-Y consensus binding element in GH4Cl but not in ROS17/2.8 cells. These results indicate that 1,25(OH)(2)D-3 may affect transcription of the human PTH gene both by competitive binding of VDR and NF-Y, and by modulating transcriptional activity of NF-Y. (C) 2005 Elsevier Inc. All rights reserved.
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