期刊
DIFFERENTIATION
卷 73, 期 4, 页码 188-198出版社
ELSEVIER SCI LTD
DOI: 10.1111/j.1432-0436.2005.00018.x
关键词
apoptosis; spermiogenesis; ectoplasmic specializations; acrosome; RAR alpha; caspase-3; retinoid signaling
资金
- NICHD NIH HHS [U01 HD060479] Funding Source: Medline
- NIDDK NIH HHS [P01 DK054075] Funding Source: Medline
The severe degeneration of the germinal epithelium and subsequent male sterility observed in mice null for the retinoic acid receptor alpha (RAR alpha) gene suggested its critical role in spermatogenesis, although the etiology and progression of these abnormalities remain to be determined. Previous studies have revealed that elongated spermatids in RAR alpha(-/-) testes were improperly aligned at the tubular lumen and did not undergo spermiation at stage VIII*. We now report a distinctive failure of step 8-9 spermatids to orient properly with regard to the basal aspect of Sertoli cells, resulting in stage VIII*-IX* tubules with randomly oriented spermatids. By in situ terminal deoxynucleotidyltransferase-mediated deoxy-UTP nick end labeling (TUNEL), we noted that elongating spermatids frequently underwent apoptosis. Immunohistochemical analysis revealed that while activated caspase-3, the primary effector caspase in the apoptotic cell death machinery, was detected in the nuclei of primary spermatocytes in the first wave of spermatogenesis and occasionally in spermatogonia of both normal and mutant testes, it was not involved in the death of elongating spermatids in RAR alpha(-/-) testes. Thus, sterility in RAR alpha(-/-) males was associated with specific defects in spermiogenesis, which may correlate with a failure in both spermatid release and spermatid orientation to the basal aspect of Sertoli cells at stage VIII* in young adult RAR alpha(-/-) testis. Further, the resulting apoptosis in elongating spermatids appears to involve pathways other than that mediated by activated caspase-3.
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