期刊
JOURNAL OF IMMUNOLOGICAL METHODS
卷 299, 期 1-2, 页码 177-188出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jim.2005.02.007
关键词
breast carcinoma; circulating cells; MUC1; cathepsin D; ELISPOT assay; metastases
In the process of metastasis, malignant cells are released from the primary tumor and migrate to specific organs via the lymphatic and blood circulation systems. These circulating tumor cells have been characterized by immunochemistry, the reverse transcription-polymerase chain reaction, and flow cytometry. Using the MCF-7 breast cancer cell line, we have developed a two-color ELISPOT assay to detect cells secreting cathepsin D protease and MUC1 glycoprotein, markers associated with the risk of metastases in breast cancer. The threshold of detection of this ELISPOT assay was one cathepsin D-or MUC1-secreting MCF7 cell per 5 ml of control blood. In 16 patients with breast carcinoma inetastases, 1 to 1940 cathepsin D- or MUC1-secreting cells per 2 X 10(7) PBMC were enumerated, whereas none were found in 11 controls. Moreover, in six patients 6-60% of MUC1-secreting cells also expressed the CXCR4 chemokine receptor, which is involved in the homing of metastatic breast cancer cells. The ELISPOT assay described here allowed us to enumerate cathepsin D- and/or MUC1-secreting cells in the MCF-7 cell line and in the peripheral blood of patients with disseminated breast cancer. The combination of the ELISPOT assay and CXCR4-positive cell sorting identified subsets of MUC1-secreting cells in the peripheral blood of these patients. (c) 2005 Elsevier B.V All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据