期刊
JOURNAL OF BACTERIOLOGY
卷 187, 期 8, 页码 2920-2925出版社
AMER SOC MICROBIOLOGY
DOI: 10.1128/JB.187.8.2920-2925.2005
关键词
-
类别
资金
- Medical Research Council [G117/519] Funding Source: Medline
- NIGMS NIH HHS [R01 GM069872] Funding Source: Medline
- Medical Research Council [G117/519] Funding Source: researchfish
- MRC [G117/519] Funding Source: UKRI
The Tat system, found in the cytoplasmic membrane of many bacteria, is a general export pathway for folded proteins. Here we describe the development of a method, based on the transport of chloramphenicol acetyltransferase, that allows positive selection of mutants defective in Tat function. We have demonstrated the utility of this method by selecting novel loss-of-function alleles of tatA from a pool of random taA mutations. Most of the mutations that were isolated fall in the amphipathic region of TatA, emphasizing the pivotal role that this part of the protein plays in TatA function.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据