期刊
RNA
卷 11, 期 4, 页码 424-436出版社
COLD SPRING HARBOR LAB PRESS, PUBLICATIONS DEPT
DOI: 10.1261/rna.7247705
关键词
5-methoxycarbonylmethyl-2-thiouridine; 5-methoxycarbonylmethyluridine; 5-carbamoylmethyluridine; Elongator complex; KTI genes
Elongator has been reported to be a histone acetyltransferase complex involved in elongation of RNA polymerase 11 transcription. In Saccharomyces cerevisiae, mutations in any of the six Elongator protein subunit (ELP1-ELP6) genes or the three killer toxin insensitivity (KTI11-KTI13) genes cause similar pleiotropic phenotypes. By analyzing modified nucleosides in individual tRNA species, we show that the ELP1-ELP6 and KTI11-KTI13 genes are all required for an early step in synthesis of 5-methoxycarbonylmethyl (mcm(5)) and 5-carbamoylmethyl (ncm(5)) groups present on uridines at the wobble position in tRNA. Transfer RNA immunoprecipitation experiments showed that the Elp1 and Elp3 proteins specifically coprecipitate a tRNA susceptible to formation of an mcm(5) side chain, indicating a direct role of Elongator in tRNA modification. The presence of mcm(5)U, ncm(5)U, or derivatives thereof at the wobble position is required for accurate and efficient translation, suggesting that the phenotypes of elp1-elp6 and kti11-kti13 mutants could be caused by a translational defect. Accordingly, a deletion of any ELP1-ELP6 or. KTI11-KTI13 gene prevents an ochre suppressor tRNA that normally contains mcm(5)U from reading ochre stop codons.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据