期刊
JOURNAL OF NEUROPHYSIOLOGY
卷 93, 期 4, 页码 2117-2126出版社
AMER PHYSIOLOGICAL SOC
DOI: 10.1152/jn.00728.2004
关键词
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资金
- NINDS NIH HHS [NS-12151] Funding Source: Medline
In the mature brain, the K+/Cl- cotransporter KCC2 is important in maintaining low [Cl-](i), resulting in hyperpolarizing GABA responses. Decreases in KCC2 after neuronal injuries result in increases in [Cl-](i) and enhanced neuronal excitability due to depolarizing GABA responses. We used the gramicidin perforated-patch technique to measure E-Cl (similar to E-GABA) in layer V pyramidal neurons in slices of partially isolated sensorimotor cortex of adult rats to explore the potential functional consequence of KCC2 downregulation in chronically injured cortex. E-GABA was measured by recording currents evoked with brief GABA puffs at various membrane potentials. There was no significant difference in E-Cl between neurons in control and undercut animals (-71.2 +/- 2.6 and -71.8 +/- 2.8 mV, respectively). However. when loaded with Cl- by applying muscimol puffs at 0.2 Hz for 60 s, neurons in the undercut cortex had a significantly shorter time constant for the positive shift in E-Cl during the Cl- loading phase (4.3 +/- 0.5 s for control and 2.2 +/- 0.4 s for undercut, P < 0.01). The positive shift in E-Cl 3 s after the beginning of Cl- loading was also significantly larger in the undercut group than in the control, indicating that neurons in undercut cortex were less effective in maintaining low [Cl-]i during repetitive activation of GAB(A), receptors. Application of furosemide eliminated the difference between the control and undercut groups for both of these measures of [Cl-](i) regulation. The results suggest an impairment in Cl- extrusion resulting from decreased KCC2 expression that may reduce the strength of GABAergic inhibition and contribute to epileptogenesis.
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