期刊
GENESIS
卷 41, 期 4, 页码 185-191出版社
WILEY
DOI: 10.1002/gene.20113
关键词
aristaless; Arx; BAC; Xenopus laevis; Xenopus; tropicalis; transgenesis; recombineering
资金
- NEI NIH HHS [R01 EY012995-04, R01 EY012995, R01 EY012995-03, R01 EY012995-05] Funding Source: Medline
- NICHD NIH HHS [HD34615] Funding Source: Medline
The aristaless-related homeobox (Arx) gene is expressed in a dynamic pattern in the developing vertebrate forebrain. We identified a bacterial artificial chromosome (BAC) containing the Xenopus tropicalis Arx gene and replaced a portion of the first coding exon with a green fluorescent protein (GFP) expression cassette by homologous recombination in bacteria (recombineering). Transgenic X. laevis embryos obtained by microinjecting the modified BAC expressed GFP in the developing forebrain in a pattern identical to that of the endogenous Arx gene. Thus, this BAC contains transcriptional regulatory elements necessary for regulating proper expression in transgenic frogs. This work demonstrates that transgenesis using recombineered BACs is a viable technique in Xenopus. (c) 2005 Wiley-Liss, Inc.
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