期刊
BIOLOGY OF REPRODUCTION
卷 72, 期 4, 页码 992-996出版社
OXFORD UNIV PRESS INC
DOI: 10.1095/biolreprod.104.036244
关键词
calcium; fertilization; gamete biology; ovum; sperm
资金
- NICHD NIH HHS [HD22732, HD042498, T32 HD007305] Funding Source: Medline
A sperm-specific phospholipase (PL) C, termed PLC zeta is proposed to be the soluble sperm factor that induces Ca2+ oscillations in mammalian eggs and, thus, initiates egg activation in vivo. We report that sperm from transgenic mice expressing short hairpin RNAs targeting PLC zeta mRNA have reduced amounts of PLC protein. Sperm derived from these transgenic mice trigger patterns of Ca2+ oscillations following fertilization in vitro that terminate prematurely. Consistent with the perturbation in patterns of Ca2+ oscillations is the finding that mating of transgenic founder males to females results in lower rates of egg activation and no transgenic offspring. These data strongly suggest that PLC zeta is the physiological trigger of Ca2+ oscillations required for activation of development.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据