期刊
BIOTECHNOLOGY LETTERS
卷 27, 期 7, 页码 471-475出版社
SPRINGER
DOI: 10.1007/s10529-005-2223-x
关键词
artificial enzyme; creatininase; cyclic amide amidohydrolase; hydantoinase; molecular imprinting
A general molecular imprinting approach is proposed to synthesize artificial enzymes to mimic the family of cyclic amide amidohydrolases which share similar active site and catalytic mechanism. The artificial enzymes were constructed by co-polymerizing 4(5)-vinylimidazole-Co2+-methacrylic acid clusters with divinylbenzene micro-spheres in the presence of corresponding substrates. The artificial enzymes mimicked creatininase and hydantoinase by showing specific affinity towards the corresponding substrates in buffer. The artificial hydantoinase also showed specific affinity towards corresponding substrate in organic solvent, and catalyzed the hydrolysis of hydantoin.
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