期刊
JOURNAL OF PLANT PHYSIOLOGY
卷 162, 期 4, 页码 457-464出版社
ELSEVIER GMBH
DOI: 10.1016/j.jplph.2004.08.005
关键词
cysteine-rich protein; exine; microspore; proteasome inhibitor; tapetum
The tapetum is a nutritive tissue of the stamen that is essential for normal microspore development. White numerous tapetal-specific genes have been identified, little information is available on the Localization and function of the proteins produced by these genes. The tapetally produced protein 5B-CRP is cysteine-rich, has a secretory signal sequence and lacks an endoplasmic reticulum retention sequence. The 5B-CRP mRNA is expressed specifically within the tapetum and accumulates from premeiosis to tetrad release. Antibodies generated against an Escherichia coli fusion protein only recognized 5B-CRP in the reduced state. The 5B-CRP was detected as a 6 kDa protein in extracts of stamens from microspore meiosis through anthesis and was also observed in extracts from dehisced pollen. In situ, 5B-CRP was localized in stamens to the tapetum and the developing microspores, from the tetrad through early free microspore stages. Based on similarity to proteins with known functions, 5B-CRP may inhibit proteasome activity within the stamen locule. (c) 2005 Elsevier GmbH. All rights reserved.
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