期刊
EXPERIMENTAL CELL RESEARCH
卷 304, 期 2, 页码 552-558出版社
ELSEVIER INC
DOI: 10.1016/j.yexcr.2004.10.036
关键词
5-bromodeoxyuridine; microarray; gene expression; cellular senescence; biomarker
We identified genes that immediately respond to 5-bromodeoxyuridine (BrdU) in SUSM-1, an immortal fibroblastic line, with DNA microarray and Northern blot analysis. At least 29 genes were found to alter gene expression greater than twice more or less than controls within 36 h after addition of BrdU. They took several different expression patterns upon addition of BrdU, and the majority showed a significant alteration within 12 h. When compared among SUSM-1, HeLa, and TIG-7 normal human fibroblasts, 19 genes behaved similarly upon addition of BrdU. In addition, 14 genes, 9 of which are novel as regards senescence, behaved similarly in senescent TIG-7 cells. The genes do not seem to have a role in proliferation or cell cycle progression. These results suggest that the early BrdU-responsive genes represent early signs of cellular senescence and can be its new biomarkers. (c) 2004 Elsevier Inc. All rights reserved.
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